Ayano Aishi (アイシア屋の aishiayano) (Yandere-chan) is the game's protagonist.
Ayano has black hair which she wears in a ponytail and her eyes are also black. Her stockings are solid black.
- In outdated versions, Ayano's persona and club said "Unknown".
- In Pre-Alpha 0.2.5b she was removed from the Stalking club.
- A dot version of her appeared in Cubedere Simulator called Cubedere-chan.